mouse anti kim 1 antibody  (R&D Systems)

Journal: Cell Reports Medicine

Article Title: Chimeric antigen receptor-induced antigen loss protects CD5.CART cells from fratricide without compromising on-target cytotoxicity

doi: 10.1016/j.xcrm.2024.101628

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Article Snippet: Mouse anti-human CD366 (TIM-3) BV421 (clone 7D3) , BD Biosciences , Cat#565562; RRID: AB_2744369.

Techniques: Enzyme-linked Immunosorbent Assay, Antibody Labeling, Western Blot, Sequencing, CRISPR, Software

Journal: Cell Reports Methods

Article Title: An autologous ex vivo model for exploring patient-specific responses to viro-immunotherapy in glioblastoma

doi: 10.1016/j.crmeth.2024.100716

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Article Snippet: Viobright FITC conjugated mouse anti-human Tim-3 , Miltenyi Biotec , Cat#130-126-004; clone F38-2E2; RRID: AB_2889789.

Techniques: Staining, Virus, Recombinant, Gene Expression, Enzyme-linked Immunosorbent Assay, Cell Viability Assay, Software

Journal: iScience

Article Title: A transfer learning framework to elucidate the clinical relevance of altered proximal tubule cell states in kidney disease

doi: 10.1016/j.isci.2024.109271

Figure Lengend Snippet: Characterization of altered proximal tubule cells (A) UMAP representation of the PT compartment from AKI and control patients. (B) Relative abundance of PT1 and PT2 clusters in AKI and control patients. (C) Expression of known injury marker genes in PT1 and PT2 clusters. (D and E) Gene-weighted density of HAVCR1 and VCAM1. (F) Enrichment score calculated by gene set enrichment analysis using Reactome pathway database (positive enrichment means an enrichment in PT2 cluster). (G) Pathway activity in PT1 and PT2 clusters (inferred from PROGENy). (H) Collagen, extracellular matrix (ecm) proteoglycan (pg) and glycoprotein (gp) scores in PT1 and PT2 clusters. (I) Potential of heat-diffusion for affinity-based trajectory embedding (PHATE) dimension reduction projecting pathway enrichment estimated by GSVA for each cell type. (J and K) Representative immunostainings of HAVCR1 (J) and VCAM-1 (K) proteins in fibrotic area. ∗∗∗p < 0.001 ∗∗∗∗p < 0.0001.

Article Snippet: mouse monoclonal anti-human HAVCR1 , clone 219211 , MAB1750; RRID: AB_2116559 ; RD Systems.

Techniques: Control, Expressing, Marker, Activity Assay, Diffusion-based Assay

Journal: iScience

Article Title: A transfer learning framework to elucidate the clinical relevance of altered proximal tubule cell states in kidney disease

doi: 10.1016/j.isci.2024.109271

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Article Snippet: mouse monoclonal anti-human HAVCR1 , clone 219211 , MAB1750; RRID: AB_2116559 ; RD Systems.

Techniques: Control, Software

Journal: iScience

Article Title: A transfer learning framework to elucidate the clinical relevance of altered proximal tubule cell states in kidney disease

doi: 10.1016/j.isci.2024.109271

Figure Lengend Snippet: Characterization of altered proximal tubule cells (A) UMAP representation of the PT compartment from AKI and control patients. (B) Relative abundance of PT1 and PT2 clusters in AKI and control patients. (C) Expression of known injury marker genes in PT1 and PT2 clusters. (D and E) Gene-weighted density of HAVCR1 and VCAM1. (F) Enrichment score calculated by gene set enrichment analysis using Reactome pathway database (positive enrichment means an enrichment in PT2 cluster). (G) Pathway activity in PT1 and PT2 clusters (inferred from PROGENy). (H) Collagen, extracellular matrix (ecm) proteoglycan (pg) and glycoprotein (gp) scores in PT1 and PT2 clusters. (I) Potential of heat-diffusion for affinity-based trajectory embedding (PHATE) dimension reduction projecting pathway enrichment estimated by GSVA for each cell type. (J and K) Representative immunostainings of HAVCR1 (J) and VCAM-1 (K) proteins in fibrotic area. ∗∗∗p < 0.001 ∗∗∗∗p < 0.0001.

Article Snippet: Immunochemistry staining were performed as followed: after antigen retrieval with pressurized heating chamber in citrate buffer pH7 or tris-EDTA pH9, 5μm tissue sections were incubated with antibodies mouse monoclonal anti-human HAVCR1 (dilution 1:250, clone 219211, RD Systems) and mouse monoclonal anti-human VCAM1 (dilution 1:25, clone 1.4C3, Invitrogen) for 1h at room temperature.

Techniques: Control, Expressing, Marker, Activity Assay, Diffusion-based Assay

Journal: iScience

Article Title: A transfer learning framework to elucidate the clinical relevance of altered proximal tubule cell states in kidney disease

doi: 10.1016/j.isci.2024.109271

Figure Lengend Snippet:

Article Snippet: Immunochemistry staining were performed as followed: after antigen retrieval with pressurized heating chamber in citrate buffer pH7 or tris-EDTA pH9, 5μm tissue sections were incubated with antibodies mouse monoclonal anti-human HAVCR1 (dilution 1:250, clone 219211, RD Systems) and mouse monoclonal anti-human VCAM1 (dilution 1:25, clone 1.4C3, Invitrogen) for 1h at room temperature.

Techniques: Control, Software